Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. edu) to be added as a trained user in iLab. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Add to Cart. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. flat meniscus), but is approx 15 mL. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Table of Contents. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Figure 6: Transcreener ADP2 FI assay principle. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. 003 fmol 0. Fill a clean plate with water. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Table 2 summarizes the results for the uniformity test. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. begin reading microplates with seven mouse clicks. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 303 unable to cal dark current. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. SpectraDrop High-Throughput Screening (HTS) Kit. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. These robust readers have been placed in labs from. We would like to show you a description here but the site won’t allow us. A new technical approach to platelet aggregation studies. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. . View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Compact and tunable absorbance microplate readers DATA SHEET. limit of detection (LLD) for ATP was estimated for each microplate reader using the . SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. SpectraMax Glo Steady-Luc Reporter Assay Kit. Please refer to the Obsolete Microplate Reader Models for more information. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Nucleic Acid and Protein Quantitation. . The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The temperature in the plate chamber is ambient and isothermal. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. 1) Select the correct plate and the wells that you want to measure. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Download PDF versions of the SpectraMax Microplate Reader User Guides. The plate reader optics are tunable for. Microplate Readers Biotek Epoch Microplate Reader. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. . It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. For more sample throughput, both readersSupplier: Molecular Devices. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. . In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The dual monochromator optics allow. microplates or in a cuvette at a. More Product Information. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. A preconfigured protocol is available in the software to. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 20 μL of Component A was added to each well of the microplate. 58% for the detection module and 2. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. The SpectraMax M2 and M2e are versatile and reliable. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SpectraMax Microplate Readers. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Self Service Available. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. We would like to show you a description here but the site won’t allow us. 100 fmol 384-well (75 µL) 0. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. . 3) This warranty. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. both cuvette-based and microplate reader applications on the same instrument. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Each sample has a discrete sample beam and reference beam so that each well is measured directly. Category 2 and 3 microplate readers use gradient heating as well. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Cell Analysis. Figure 1. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The powerful analysis tools in the software generate a standard curve and calculated data. SpectraMax iD5 Multi-Mode Microplate Reader. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. 0 out of 5 $ 2,999-20%. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. SpectraMax Spectrofluorometers. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. Find regional support. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Materials. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Avoid losing high signal. Five preferred detection modes. Add to Cart. Sensitive fluorescent quantitation of DNA with the Quant-iT. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Examples: “Press carefully to engage,” “ Do not press down. These lab workhorses are used in drug discovery, research, bioassay. B: Selection of Excitation Wavelength. 0 out of 5 $ 6,499. Create custom fluorescence assays without having to purchase expensive filter sets. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Contact our support team with your questions regarding Molecular Devices products and applications. In addition, the SpectraMax iD5 can be expanded to. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Cuvette Microplate wells. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Products. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Microplate Spectrophotometers. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. 72 MB. SpectraMax Spectrofluorometers. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 1. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 0 out of 5 $ 5,999. Turn the reader off by pulling the power cord. 5 87. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Figure 1. Type: Clear: Add to Cart. Assays can be better optimized by scanning across. 0 out of 5 $ 11,999. 6% for the on-board optics (monochromators). Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. All stand-alone instrument functions can be controlled using the software. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. Wide range of applications The VersaMax reader covers. 313 reference gain check fail. The dual monochromator optics allow. The plate reader can measure multiple readouts, including UV and visible absorbance. Request a Quote!Introduction. Supplier: Molecular Devices. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Step 2: Launch SoftMax Pro Software. In . The calculated LOQ values were 0. View the Molecular Devices website. SpectraMax i3x Multi-Mode Detection Platform. We use Spectramax M5 microplate reader in our lab. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Transcreener ® ADP. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. flexibility of monochromator-based systems. ATP standard curves read on SpectraMax microplate readers. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. The screw remains inside the instrument. 305 reference level saturation. Using the. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. 0 out of 5 $ 5,999. Read More. 1. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. The advanced optical and. zip. 314 low lamp level warning. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. 1. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. Introduction. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. These lab workhorses are used in drug discovery. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Blank subtraction and standard curve fitting were done automatically by the software. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. All . settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. Brochure. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 2. Results are shown in Table 1. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SAN JOSE, CA — Molecular Devices, LLC. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Wide range of applications. Omnia® Compatible Microplate Reader Documentation Version No. Download PDF. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Download PDF. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Tecan Infinite Microplate Reader. Also see:. Lift up the lid over the chamber to insert or remove a cuvette. Table 1. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. . 2 only: SpectraMax Mini Update. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Absorbance (optical density, OD) was read at 570 nm and 605. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. Highly customizable low volume. 1. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Figure 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. . Detailed specifications of each model reader are reported in Section. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). For readers without a cuvette port, simply check the box next to ‘PathCheck’. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. 0 out of 5 $ 5,999. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Add to Cart. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The Gemini EM can sample a variety of formats, from 6- to 384-well. 300 µL of Detergent Compatible Bradford reagent was added to each well. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The system uses two scanning monochromators to determine the optimal excitation and emission settings. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 306 plate air cal fail,low light. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Total volume (except deep. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. microplate reader. both cuvette-based and microplate reader applications Microplate Readers. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Cell Cytotoxicity assay procedure. Cell Cytotoxicity assay procedure. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. With optimized reagents, option to operate the. 2. Compact designs. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. It was also read on the monochromator-based. The guide also covers the software features, the specifications, and the accessories of the systems. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. No accessories are needed and SoftMax® Pro Software. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Figure 1. For a higher . Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. View the Molecular Devices website. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Figure 3. \ Figure 3. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT.